10+ puasp-attb vector
Gateway cloning using pENTR-D-AthHEN1DmeCO entry vector and pUASp-FMW-attB destination vector backbone_origin. The pUAST-C-TAP-BD expression vector Stapleton was cut with NotI and XbaI to release a 256bp fragment containing loxP chloramphenicol promoter and splice acceptor sequences.
Frontiers The Tudor Domain Containing Protein Kotsubu Cg9925 Localizes To The Nuage And Functions In Pirna Biogenesis In D Melanogaster
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. To recombine an Entry clone with a Destination clone. For this reason the pUASTattB vector system is designed to be used with Drosophila lines carrying attP landing sites within their genome. The other vector referred to as the helper plasmid encodes for the φC31 integrase.
Gateway LR Clonase Enzyme Mix. Miniprep DNA of an Entry clone containing your ORF. When the pUASTattB and the φC31 helper plasmids are co-injected into cells containing attP.
Miniprep DNA of the desired Destination vector. Stefan Ameress lab contains the insert Hen1 and is published in Nat Methods. Sutter Instrument transformation vector puasp k10 attb Transformation Vector Puasp K10 Attb supplied by Sutter Instrument used in various techniques.
SnapGene Viewer is free software that allows molecular biologists to create browse and share richly annotated sequence files. Gain unparalleled visibility of your plasmids DNA and protein. This 167bp C-terminus BD cassette was inserted into the linearized pUAST vector by overnight ligation.
The resulting N-terminus BD-adapted pUAST vector was linearized again by cutting it. 86100 based on 1. When publishing experiments using materials obtained from the DGRC please follow the citation guidelines on the materials stock page including citing the.
Plasmid pUASp-FMW-attB-AthHen1 from Dr.
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Asf1 Is Required To Load Histones On The Hira Complex In Preparation Of Paternal Chromatin Assembly At Fertilization Epigenetics Chromatin Full Text
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